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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
05/12/2016 |
Actualizado : |
07/12/2018 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
CARLSON, J. S.; GIANNITTI, F.; VALKIÜNAS, G.; TELL, L. A.; SNIPES, J.; WRIGHT, S.; CORNEL, A. J. |
Afiliación : |
JENNY S. CARLSON, Mosquito Control Research Laboratory, Department of Entomology and Nematology, Kearney Agriculture Center, University of California; FEDERICO GIANNITTI, University of Minnesota, Saint Paul, MN, USA; INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; University of California, Davis, CA, USA; GEDIMINAS VALKIÜNAS, Nature Research Centre, Akademijos 2; LISA A. TELL, Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California; JOY SNIPES, Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California; STAN WRIGHT, Sacramento-Yolo Mosquito Vector and Control District; ANTHONY J. CORNEL, Mosquito Control Research Laboratory, Department of Entomology and Nematology, Kearney Agriculture Center, University of California; Vector Genetics Laboratory, Department of Pathology, Microbiology and Immunology, University of California. |
Título : |
A method to preserve low parasitaemia Plasmodium-infected avian blood for host and vector infectivity assays. |
Complemento del título : |
Methodology. |
Fecha de publicación : |
2016 |
Fuente / Imprenta : |
Malaria Journal, 2016, v.15 (5): Article number 1198. OPEN ACCESS |
ISSN : |
1475-2875 |
DOI : |
10.1186/s12936-016-1198-5 |
Idioma : |
Inglés |
Notas : |
Article history: Received 08 July 2015 // Accepted 01 March 2016 //First Online 11 March 2016. |
Contenido : |
ABSTRACT.
Background: Avian malaria vector competence studies are needed to understand more succinctly complex avian parasite-vector-relations. The lack of vector competence trials may be attributed to the difficulty of obtaining gametocytes for the majority of Plasmodium species and lineages. To conduct avian malaria infectivity assays for those Plasmodium spp. and lineages that are refractory to in vitro cultivation, it is necessary to obtain and preserve for short periods sufficient viable merozoites to infect naïve donor birds to be used as gametocyte donors to infect mosquitoes. Currently, there is only one described method for long-term storage of Plasmodium spp. - infected wild avian blood and it is reliable at a parasitaemia of at least 1 %. However, most naturally infected wild-caught birds have a parasitaemia of much less that 1 %. To address this problem, a method for short-term storage of infected wild avian blood with low parasitaemia (even ?0.0005 %) has been explored and validated. Methods: To obtain viable infective merozoites, blood was collected from wild birds using a syringe containing the anticoagulant and the red blood cell preservative citrate phosphate dextrose adenine solution (CPDA). Each blood sample was stored at 4°C for up to 48 h providing sufficient time to determine the species and parasitaemia of Plasmodium spp. in the blood by morphological examination before injecting into donor canaries. Plasmodium spp. - infected blood was inoculated intravenously into canaries and once infection was established, Culex stigmatosoma, Cx. pipiens and Cx. quinquefasciatus mosquitoes were then allowed to feed on the infected canaries to validate the efficacy of this method for mosquito vector competence assays. Results: Storage of Plasmodium spp. - infected donor blood at 4°C yielded viable parasites for 48 h. All five experimentally-infected canaries developed clinical signs and were infectious. Pathologic examination of three canaries that later died revealed splenic lesions typical of avian malaria infection. Mosquito infectivity assays demonstrated that Cx. stigmatosoma and Cx. pipiens were competent vectors for Plasmodium cathemerium. Conclusions: A simple method of collecting and preserving avian whole blood with malaria parasites of low parasitaemia (?0.0005 %) was developed that remained viable for further experimental bird and mosquito infectivity assays. This method allows researchers interested in conducting infectivity assays on target Plasmodium spp. to collect these parasites directly from nature with minimal impact on wild birds.
© 2016 Carlson et al. MenosABSTRACT.
Background: Avian malaria vector competence studies are needed to understand more succinctly complex avian parasite-vector-relations. The lack of vector competence trials may be attributed to the difficulty of obtaining gametocytes for the majority of Plasmodium species and lineages. To conduct avian malaria infectivity assays for those Plasmodium spp. and lineages that are refractory to in vitro cultivation, it is necessary to obtain and preserve for short periods sufficient viable merozoites to infect naïve donor birds to be used as gametocyte donors to infect mosquitoes. Currently, there is only one described method for long-term storage of Plasmodium spp. - infected wild avian blood and it is reliable at a parasitaemia of at least 1 %. However, most naturally infected wild-caught birds have a parasitaemia of much less that 1 %. To address this problem, a method for short-term storage of infected wild avian blood with low parasitaemia (even ?0.0005 %) has been explored and validated. Methods: To obtain viable infective merozoites, blood was collected from wild birds using a syringe containing the anticoagulant and the red blood cell preservative citrate phosphate dextrose adenine solution (CPDA). Each blood sample was stored at 4°C for up to 48 h providing sufficient time to determine the species and parasitaemia of Plasmodium spp. in the blood by morphological examination before injecting into donor canaries. Plasmodium spp. - infected blood was inoculated int... Presentar Todo |
Palabras claves : |
ANIMAL EXPERIMENT; AVIAN MALARIA; BIRD INOCULATION; BLOOD PRESERVATION; CULEX spp. VECTORS; EXPERIMENTAL INFECTION; PATHOLOGY; PLASMODIUM CATHEMERIUM. |
Thesagro : |
MODELOS ANIMALES. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/12151/1/s12936-016-1198-5.pdf
https://malariajournal.biomedcentral.com/articles/10.1186/s12936-016-1198-5
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Marc : |
LEADER 03718naa a2200337 a 4500 001 1056201 005 2018-12-07 008 2016 bl uuuu u00u1 u #d 022 $a1475-2875 024 7 $a10.1186/s12936-016-1198-5$2DOI 100 1 $aCARLSON, J. S. 245 $aA method to preserve low parasitaemia Plasmodium-infected avian blood for host and vector infectivity assays.$h[electronic resource] 260 $c2016 500 $aArticle history: Received 08 July 2015 // Accepted 01 March 2016 //First Online 11 March 2016. 520 $aABSTRACT. Background: Avian malaria vector competence studies are needed to understand more succinctly complex avian parasite-vector-relations. The lack of vector competence trials may be attributed to the difficulty of obtaining gametocytes for the majority of Plasmodium species and lineages. To conduct avian malaria infectivity assays for those Plasmodium spp. and lineages that are refractory to in vitro cultivation, it is necessary to obtain and preserve for short periods sufficient viable merozoites to infect naïve donor birds to be used as gametocyte donors to infect mosquitoes. Currently, there is only one described method for long-term storage of Plasmodium spp. - infected wild avian blood and it is reliable at a parasitaemia of at least 1 %. However, most naturally infected wild-caught birds have a parasitaemia of much less that 1 %. To address this problem, a method for short-term storage of infected wild avian blood with low parasitaemia (even ?0.0005 %) has been explored and validated. Methods: To obtain viable infective merozoites, blood was collected from wild birds using a syringe containing the anticoagulant and the red blood cell preservative citrate phosphate dextrose adenine solution (CPDA). Each blood sample was stored at 4°C for up to 48 h providing sufficient time to determine the species and parasitaemia of Plasmodium spp. in the blood by morphological examination before injecting into donor canaries. Plasmodium spp. - infected blood was inoculated intravenously into canaries and once infection was established, Culex stigmatosoma, Cx. pipiens and Cx. quinquefasciatus mosquitoes were then allowed to feed on the infected canaries to validate the efficacy of this method for mosquito vector competence assays. Results: Storage of Plasmodium spp. - infected donor blood at 4°C yielded viable parasites for 48 h. All five experimentally-infected canaries developed clinical signs and were infectious. Pathologic examination of three canaries that later died revealed splenic lesions typical of avian malaria infection. Mosquito infectivity assays demonstrated that Cx. stigmatosoma and Cx. pipiens were competent vectors for Plasmodium cathemerium. Conclusions: A simple method of collecting and preserving avian whole blood with malaria parasites of low parasitaemia (?0.0005 %) was developed that remained viable for further experimental bird and mosquito infectivity assays. This method allows researchers interested in conducting infectivity assays on target Plasmodium spp. to collect these parasites directly from nature with minimal impact on wild birds. © 2016 Carlson et al. 650 $aMODELOS ANIMALES 653 $aANIMAL EXPERIMENT 653 $aAVIAN MALARIA 653 $aBIRD INOCULATION 653 $aBLOOD PRESERVATION 653 $aCULEX spp. VECTORS 653 $aEXPERIMENTAL INFECTION 653 $aPATHOLOGY 653 $aPLASMODIUM CATHEMERIUM 700 1 $aGIANNITTI, F. 700 1 $aVALKIÜNAS, G. 700 1 $aTELL, L. A. 700 1 $aSNIPES, J. 700 1 $aWRIGHT, S. 700 1 $aCORNEL, A. J. 773 $tMalaria Journal, 2016$gv.15 (5): Article number 1198. OPEN ACCESS
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1. | | LISTUR, B.; BALDIVIA, G.; CONIBERTI, A.; MARTÍN, V.; BOIDO, E.; MEDINA, K.; CARRAU, F.; DELLACASSA, E.; FARIÑA, L. Estrategias combinadas para la obtención de un vino Tannat con un contenido moderado de alcohol. [Combined strategies for obtaining a Tannat wine with moderate alcohol content.]. [conference proceedings]. BIO Web of Conferences. 2023, Volume 68, Article 02045. 44th World Congress of Vine and Wine, OIV 2023, Cadiz/Jerez, 5 - 9 June 2023. https://doi.org/10.1051/bioconf/20236802045 Document type: Conference Paper, Gold Open Access, Green Open Access. -- Publisher: EDP Sciences. -- Funding: Proyecto CSIC - Udelar I+D - 2020 "Estrategias para la obtención de un vino Tannat con un contenido moderado de alcohol". Beca...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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2. | | FARIÑA, L.; BOIDO, E.; ARES, G.; GONZALEZ, N.; LADO, J.; CURBELO, R.; ALMEIDA, L.; MEDINA, K.; CARRAU, F.; DELLACASSA, E, Solid phase microextraction for the characterization of food aroma and particular sensory defects. (Chap.6) In: ACS Symposium Series, 2023, Volume 1433, Pages 299 - 325. Flavors and Fragrances in Food Processing: Preparation and Characterization Methods. Balakrishnan P., Gopi S. (editors). doi: https://doi.org/10.1021/bk-2022-1433.ch006 (ACS Symposium Series; Volume 1433). Chapter book history: Publication Date (Web):December 28, 2022 -- Corresponding author: Dellacassa, E.; Laboratorio de Biotecnología de Aromas, Departamento de Química Orgánica, Facultad de Química, Universidad de la República, Av....Tipo: Capítulo en Libro Técnico-Científico |
Biblioteca(s): INIA Las Brujas. |
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3. | | CARRAU, F.; BOIDO, E.; GAGGERO, C.; MEDINA, K.; FARIÑA, L.; DISEGNA, E.; DELLACASSA, I. Vitis vinifera Tannat, chemical characterization and functional properties. Ten years of research. In: Multidisciplinary Approaches on Food Science and Nutrition for the XXI Century. Research Signpost/ Transworld Res. Network. Rosana Filip (editor), Kerala, India, 2011. pp.53-71. ISBN: 978-81-7895-504-9 Correspondence/Reprint request: Dr. E. Dellacassa, Cátedra de Farmacognosia y Productos Naturales, Facultad de Química, Universidad de la República, Gral. Flores 2124, 11800-Montevideo, Uruguay. E-mail: edellac@fq.edu.uyTipo: Capítulo en Libro Técnico-Científico |
Biblioteca(s): INIA Las Brujas. |
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4. | | FARIÑA, L.; CARRAU, F.; BOIDO, E.; DISEGNA, E.; MEDINA, K.; LLORET, A.; LORENZO, D.; VERSINI, G.; VERSINI, G.; DELLACASSA, E. Caracterización quimiotaxonómica de la Vitis Vinífera L. var. Tannat: importancia de las fracciones glicosidadas en la uva y el vino. Sección I: Metodología y Ecofisiología. In: Jornadas, 13as. (3-8 Feb. 2003 : Montevideo, UY).; GESCO. Libro de actas / GESCO 2003. Uruguay. Montevideo : Banco de Seguros del Estado, 2003. p. 49-50 Organizadores de las XIII Jornadas del GESCO: Milka Ferrer (Fac.Agron. UDELAR); Edgardo Disegna (INIA); Graciela Calero y Emilia Pattarino (Escuela de Vitivinicultura); Julio Borsani, Jorge Fernández y Enrique Mirazo (FUCREA); Ana...Tipo: Trabajos en Congresos/Conferencias |
Biblioteca(s): INIA Las Brujas. |
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5. | | DISEGNA, E.; BOIDO, E.; CARRAU, F.; FARIÑA, L.; MEDINA, K.; MÉNDEZ, M.; RODRIGUEZ, P.; DELLACASSA, E. Efectos de la aplicación del regulador de crecimiento 3,5-Dioxo-4Propionilciclohexancarboxilato de calcio (BAS 125) en la producción de uvas, composición del vino y aroma del cv. Tannat (Primera comunicación). Sección I: Metodología y Ecofisiología. In: Jornadas, 13as. (3-8 Feb. 2003 : Montevideo, UY).; GESCO. Libro de actas / GESCO 2003. Uruguay. Montevideo : Banco de Seguros del Estado, 2003. p. 51-55 Organizadores de las XIII Jornadas del GESCO: Milka Ferrer (Fac.Agron. UDELAR); Edgardo Disegna (INIA); Graciela Calero y Emilia Pattarino (Escuela de Vitivinicultura); Julio Borsani, Jorge Fernández y Enrique Mirazo (FUCREA); Ana...Tipo: Trabajos en Congresos/Conferencias |
Biblioteca(s): INIA Las Brujas. |
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